NOTCH3 Knockout cell line(Huh-7)

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NOTCH3 Knockout cell line(Huh-7) Order

NOTCH3 Knockout cell line(Huh-7)
Catalog#
YKO-H415
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NOTCH3 Knockout cell line(Huh-7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NOTCH3 Knockout cell line (Huh-7)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
NOTCH3
Gene ID
4854
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript NOTCH3-201)
Validation: PCR+Sanger Sequencing
NOTCH3 Knockout cell line(Huh-7) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HuH-7		 STR Info (Cell bank)
Cell Line:HuH-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 11111111
D16S539 10101010
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NOTCH3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HuH-7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NOTCH3 gene knockout NOTCH3 Knockout cell line (Huh-7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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